can i use EDTA instead of TEMED for SDS PAGE ?

Pow Joshi via (by pow.joshi from
Wed May 28 13:19:38 EST 2008

2008/5/28 Aawara Chowdhury <aawara from>:

> In <6I5%j.233$k67.12 from newsfe02.lga>,
>  DK <dk from> wrote:
> > In article <mailman.233.1211945543.3533.methods from>,
> hroychow from wrote:
> >>Before we could pour a single acrylamide gel (tube gel at that time) in
> >>one of my early graduate labs (I was fresh out of my undergad), our
> >>supervisor made us research and jointly write a short paper PAGE.
> Although
> >>my friend and I were not too thrilled at that time, in retrospect I am
> >>immensely thankful for that.
> >
> > Did it involve the understanding of how discontinuous EF works?
> > I have yet to meet a graduate student that was taught it.
> I was ....
> AC

 so was I ....  biochem 101.... any book including Lehninger, Stryer, Voet
and Voet, give a detailed and lucid explanation.... sometimes I guess though
folks love to re-invent the wheel....or begin where life began, with EDTA or
with Oparin's coacervates :), and perhaps call it a different name "Blobs"?
(kidding) ...

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