can i use EDTA instead of TEMED for SDS PAGE ?

Pow Joshi via methods%40net.bio.net (by pow.joshi from gmail.com)
Thu May 29 08:58:24 EST 2008


2008/5/28 DK <dk from no.email.thankstospam.net>:

> In article <mailman.243.1212019971.3533.methods from net.bio.net>, "Pow Joshi"
> <pow.joshi from gmail.com> wrote:
> >2008/5/28 Aawara Chowdhury <aawara from pontiff-playground.org>:
> >
> >> In <6I5%j.233$k67.12 from newsfe02.lga>,
> >>  DK <dk from no.email.thankstospam.net> wrote:
> >>
> >> > In article <mailman.233.1211945543.3533.methods from net.bio.net>,
> >> hroychow from nmsu.edu wrote:
> >> >>Before we could pour a single acrylamide gel (tube gel at that time)
> in
> >> >>one of my early graduate labs (I was fresh out of my undergad), our
> >> >>supervisor made us research and jointly write a short paper PAGE.
> >> Although
> >> >>my friend and I were not too thrilled at that time, in retrospect I am
> >> >>immensely thankful for that.
> >> >
> >> > Did it involve the understanding of how discontinuous EF works?
> >> > I have yet to meet a graduate student that was taught it.
> >>
> >> I was ....
> >>
> >> AC
> >
> > so was I ....  biochem 101.... any book including Lehninger, Stryer, Voet
> >and Voet, give a detailed and lucid explanation....
>
> None of the books you mention does that ...


well.... I remember that they did ... if we're speaking of IEF... else I
made a mistake.

>
>
> DK
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