can i use EDTA instead of TEMED for SDS PAGE ?
(by pow.joshi from gmail.com)
Thu May 29 17:04:34 EST 2008
2008/5/29 DK <dk from no.email.thankstospam.net>:
> In article <mailman.251.1212073353.3533.methods from net.bio.net>, "Pow Joshi"
> <pow.joshi from gmail.com> wrote:
> >2008/5/28 DK <dk from no.email.thankstospam.net>:
> >> In article <mailman.243.1212019971.3533.methods from net.bio.net>, "Pow
> >> <pow.joshi from gmail.com> wrote:
> >> > so was I .... biochem 101.... any book including Lehninger, Stryer,
> >> >and Voet, give a detailed and lucid explanation....
> >> None of the books you mention does that ...
> >well.... I remember that they did ... if we're speaking of IEF... else I
> >made a mistake.
> I was wrong. Just checked all three. Voet and Voet does it. Not
> in a way that's understandable by any one who does not understand
> Ohm's law but it does nevertheless. It's not what can be called
> detailed and lucid explanation but at least it's there.
> Stryer (the best biochemistry textbook for 101, IMHO) makes
> no mention of discontinuous electrophoresis at all.
> New Lehninger not only does not mention discontinuous EF but
> also does atrocious job describing EF in general and I think
> makes several errors doing it:
> - "Migration may also be affected by protein shape". (It's not
> "may be", it's "is").
> - "In EF, the force moving the macromolecule is the electric
> potential, E" (wrong, driving force is an electric field).
> - "The electrophoretic mobility of the molecule, mu, is the
> ratio of the velocity of the particle molecule, V, to the electrical
> potential E. (It's "electric". Wrong. E is electric field strength).
> - "mixture of low molecular weight organic acids and bases
> (ampholytes, p.81)". (How about writing properly: mixture of
> zwitterions having different pI values that cover desired pH
> - Why smaller popypeptides are moving more rapidly is never
> explained. The statement that precedes SDS-PAGE serves
> only to confuse: "The polyacrylamide gel acts as a molecular
> sieve, slowing migration of proteins approximately to their
> charge- to-mass ratio". (Umm, but the whole idea of SDS
> is to make approximately the same charge to mass ratio
> for all proteins!).
Thanks DK ... I used to own Voet and Voet .... and refer the other two .....
yes, I agree that the other two books were'nt my favorites .... but then
other folks usedto swear by them ..so, well...
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