protocol for liposome preparation

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Sat Nov 29 15:22:34 EST 2008


Hi Michael,

you might try this: dissolve PC in EtOH and inject into 20 to 50 fold
volume of buffer with an insulin syringe (or the narrowest needle you
can get) while vortexing. If the EtOH might cause problems downstream,
dialyze against buffer.

Worked well for PC/Ceramide vesicles for me.

best regards,

Wo


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