Inert "carrier" for small numbers of tissue culture cells during centrifugation?

Nick Theodorakis via methods%40net.bio.net (by nick_theodorakis from hotmail.com)
Sun Oct 5 19:26:21 EST 2008


Wendy Ingram wrote:
> Does anyone know of an inert substance, home made or commercial, that
> can be added to mammalian cell cultures when spinning? I'm looking for a
> "carrier" that won't harm live cells, but provides "bulk" to the cell
> pellet when it would otherwise be very tiny. The goal is to increase
> recovery when there are very few cells... Hopefully using something that
> looks different to cells under the microscope when pellets are
> eventually resuspended. I guess I'm looking for the live cell equivalent
> of "pellet paint", which is used as a carrier for DNA work. Does anyone
> know if there is there something similar out there for tissue culture use?
> 
> Regards,
>         Wendy
> 

As a wild guess, I would look into colored microspheres.

Nick

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Nick Theodorakis
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