Helping to isolate DNA that very viscosity

[chovek69]

The simple solution would be to try with less starting material.
Increase also incubation time with Prot K or its amount. Undegisted
proteins could contribute to viscosity.

The greenish color can be removed (or at least lessen) by doing one
extraction with only phenol before the phenol/ chloroform step.

The precipitation should be performed by using less centrifugation
speed (say 10 000 rpm instead of 13k rpm). Washing with 70% ethanol
however is on max speed.

Hope this helps



TRAN HO QUANG wrote:
> Hello,
>
> We have isolated DNA from leaves of native species by the CTAB method but we are facing problems: the solution at early and late steps are very viscosity and green colors. Measuring DNA concentration on NanoDrop is nothing.
>
>
> Does anyone has experiences on this issue?. I would like to appreciated to get right comments.
>
> Digifellow.