How reliable is ligation independent cloning ?

ab via methods%40net.bio.net (by anirbn from gmail.com)
Thu Oct 9 23:02:03 EST 2008


Dear all,

I have to generate a large number of constructs for different genes
and am considering ligation independent cloning. However, someone told
me today that ligation independent cloning is not that reliable and
can give rise to insertions/mutations . Does that agree with your
experience ? Will you please share ? What will be your advice ?

Thanks a ton in advance.

AB.


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