Detection enhancer in Ambion AgPath 1-step real time RTPCR kit

[Hamel, Andre (MAFRI)]

Hi,

I have some questions/concerns about Ambion's AgPath 1-step real time
RTPCR kit & their "detection enhancer" ("DE").

1 - Does anyone have experience with the performance of comparing with &
without DE?

2 - Has anyone obtained any written information from folks at ABI and/or
Ambion regarding NOT using the DE with their kit?

3 - Does anyone have any idea as to what the DE consists of? - for
example is it simply betaine?

Thanks,

Andre Hamel, Scientist (MSc)
Virology, Veterinary Diagnostic Services
Manitoba Agriculture, Food and Rural Initiatives (MAFRI)
545 University Crescent, Winnipeg, Manitoba, Canada R3T 5S6
Ph:(204)945-7651;   Fax:(204)945-8062;
Andre.Hamel from gov.mb.ca