A question about pET system

Qingwu Shen via methods%40net.bio.net (by shenq from purdue.edu)
Thu Oct 23 15:17:22 EST 2008


Hi everybody,

I have a question about pET system. As you know, pET vectors do not have lacZ ¦Á-
peptide and are not applicable to blue/white screening. However, why the colonies 
still become blue on plate when XL1 blue transformed with pET28 basic vector is 
grown for more than 36 hours? Thank you very much.

shen



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