Protein elution from PVDF membrane

jh via methods%40net.bio.net (by biocjh from gmail.com)
Thu Oct 23 21:40:45 EST 2008


maybe high salt solution will help.

Jianhao

2008/10/23 Amit <amitsk84 from yahoo.co.in>:
> Dear Sir/ Madam,,
>
> I would like to state that i am a research assistant here in India, at post graduate institute for medical sciences and research, Chandigarh. Presently we are working on some protein profiling and some work with reverse genetics. In this process we are in need of some protocols or methods that can work with binding of some antibodies (in serum form) on either nitrocellulose membrane or PVDF membrane. After binding of the antibodies we are looking for the elution  of proteins  of interest  from the membrane. in this process  we are looking for  some  standardized protocol or methods.
> I got this mail address a link from the website www.bio.net.com
>
> I will very grateful to you if you can suggest some methods or mail some protocol for this.
>
> thanking you.
>
> Best regards,
> Amit Khatri
> Research Assistant
> PGIMER, Chandigarh
> India
>
>
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