RT-PCR question

chovek69 via methods%40net.bio.net (by ivanoov from gmail.com)
Fri Oct 24 05:04:05 EST 2008


Dear experts,

I am relatively new to the RT-PCR world and just would like to hear
some suggestions about some basic practices:

Usually I come up with the RNAs in the evening so what is better: to
run RT-PCR with hexamers overnight (cDNA stay at room temp whole
night) or to freeze the RNA at -80C and do the RT-PCR on the next
day ?

I am to run PCRs for viral detection (low-copy number) in ticks.

Thanks


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