RT-PCR question

[chovek69]

Dear experts,

I am relatively new to the RT-PCR world and just would like to hear
some suggestions about some basic practices:

Usually I come up with the RNAs in the evening so what is better: to
run RT-PCR with hexamers overnight (cDNA stay at room temp whole
night) or to freeze the RNA at -80C and do the RT-PCR on the next
day ?

I am to run PCRs for viral detection (low-copy number) in ticks.

Thanks