RT-PCR question

schisler from gmail.com via methods%40net.bio.net (by schisler from gmail.com)
Mon Oct 27 14:55:34 EST 2008


On Oct 24, 6:04 am, chovek69 <ivan... from gmail.com> wrote:
> Dear experts,
>
> I am relatively new to the RT-PCR world and just would like to hear
> some suggestions about some basic practices:
>
> Usually I come up with the RNAs in the evening so what is better: to
> run RT-PCR with hexamers overnight (cDNA stay at room temp whole
> night) or to freeze the RNA at -80C and do the RT-PCR on the next
> day ?
>
> I am to run PCRs for viral detection (low-copy number) in ticks.
>
> Thanks

If you run your cDNA synthesis reaction using a thermocycler, just add
a final hold at 4 degrees and it will be ready for you in the
morning.


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