Problems using pGEX - other expression vector?

Nikola Wenta via methods%40net.bio.net (by Nikola.Wenta from nottingham.ac.uk)
Thu Oct 30 18:01:50 EST 2008


 Hi!
Maybe it's codon usage? Then you should use a codon-optimized strain
like Rosetta. If it's the vector, why not trying pASK-IBA; they also
provide the same restriction sites, and they provide a Strep-tag which
makes protein easier and yields in more pure than GST.
Cheers
Niko

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