Larger numbers of DNA extractions...

Glenn Dunshea via methods%40net.bio.net (by glenn.dunshea from gmail.com)
Tue Sep 2 19:14:28 EST 2008


There are various 96 well format cheap extraction methods that have been
published in the last couple of years in molecular ecology notes. For
example:

A high-throughput protocol for extracting high-purity
genomic DNA from plants and animals (2008)
R. WHITLOCK, H. HIPPERSON, M. MANNARELLI and T. BURKE

See also
Elphinstone et al. Same journal a year or two before

Cheers,
Glenn

On Wed, Sep 3, 2008 at 9:21 AM, Rory O'Brien <
rory.obrien from stonebow.otago.ac.nz> wrote:

> Can anyone suggest an appropriate methodology for extracting DNA from a
> large number (1,000ish) of stored buffy coats?
>
> Maybe somebody has a 96 well plate DNA extraction protocol they might care
> to share; the commercial ones are sooo expensive!
>
> Downstream application is a simple PCR allelic discrimination assay.
>
> Many thanks,
>
> - Rory
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