BAC Protocol

[Christian Praetorius]

"sanjiban chakrabarty" <sanjiban.c from gmail.com> wrote:

>I am working with BAC DNA and I have faced lot of problem with BAC minipreps
>as the yield is always very low and the BAC DNA is contaminated with E Coli
>genomic DNA.

We have either been using a modified protocol for the Qiagen Midi prep
Kit (http://www1.qiagen.com/literature/render.aspx?id=450&tp=9) or the
PhasePrep BAC DNA Kit from Sigma (#NA0100). Yields were usually quite
low, but since we prepared the BACs only to transfer them between
different bacterial strains, this was ok for us.

Christian

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X-no-Sig: yes