E. coli strain request
(by bmacgreg from unc.edu)
Fri Sep 5 14:00:28 EST 2008
I can't recommend a particular strain, but you can search for strains
by genotype at CGSC:
Sorry if you've already looked there!
> We wish to use lactose (and not IPTG) to induce expression of genes
> into pGEX vectors (under control of the lac operator) for large scale
> protein purification. To utilise lactose, as opposed to IPTG,
> requires an
> E. coli strain that has a wild type lac operon, wild type gal operon
> ideally ompT and lon defective to prevent protease degradation of the
> expressed gene.
> Anyone out there have such an E.coli strain or suggestions? Most lab
> strains appear to be mutated in the lac and/or gal operons.
> Mick Jones
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