pET15b (Novagen) IPTG induction

E. Lis via methods%40net.bio.net (by elz.lis from gmail.com)
Mon Sep 8 06:00:24 EST 2008


It is possible that your protein is present in inclusion bodies, and 
lysozyme and sonication will not disrupt those. Have you tried lysis by 
8M urea?
You could also try running entire bacterial cells suspended in SDS-PAGE 
sample buffer (as 10 min in sample buffer at 100^C should do the trick).



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