info abt linker in GFP fusion protein

[Itisam Sarangi]

Hi all,
I am new to the cell biology field. I am presently trying to tag eGFP to my
protein of intrest in Pmyc vector. I am trying to prepare a vector with MCS
before and after eGFP.

what shd be the minimum length of linker so tht I can see GFP in cell after
transfection?
which one is better C -terminal or N terminal tagged?
What if I give MCS before or after GFP so tht gfp remains in frame still I
can put any other protein? where can I find regarding MCS

any help in this matter will be helpful.


Thanking you

-- 
Itisam Sarangi