Removal of Melanin from DNA/RNA
Selvam-Akumulla Philomina
via methods%40net.bio.net
(by Philomina.Selvam from usz.ch)
Thu Sep 18 08:31:54 EST 2008
Respected Sir
I am Mrs.Selvam working in Dermatology Skin Cancer Research.
Our major projects are Melanoma and Lymphoma. I want to try
GITC to remove melanin from biopsie, later extract DNA for
HLA Typing.
Can you help me right recepie of GITC, I have the following
recepie for GITC: 5.0M Guanidine Isothiocyanate
8% Mercap-toethanol
10mM EDTA
50mM Tris
pH 7.5
weather it is right one or not. attachment of protokol.
Thanks in advance
With Regards
Philomina Selvam
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