Removal of Melanin from DNA/RNA

Selvam-Akumulla Philomina via methods%40net.bio.net (by Philomina.Selvam from usz.ch)
Thu Sep 18 08:31:54 EST 2008


Respected Sir

I am Mrs.Selvam working in Dermatology Skin Cancer Research.
Our major projects are Melanoma and Lymphoma. I want to try
GITC to remove melanin from biopsie, later extract DNA for 
HLA Typing.

Can you help me right recepie of GITC, I have the following
recepie for GITC: 5.0M Guanidine Isothiocyanate
                         8% Mercap-toethanol
                         10mM EDTA
                         50mM Tris
                         pH 7.5
weather it is right one or not.  attachment of protokol.

Thanks in advance

With Regards
Philomina Selvam






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