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satheesh kumar via methods%40net.bio.net (by sayasatheesh from gmail.com)
Fri Sep 19 06:44:40 EST 2008


Sir,


 Im satheesh from India. Im doing work on finding a genetic diversity on
different coffea species using RAPD marker analysis. For the RAPD analysis
require a Taq Polymerase enzyme. We better to optimization of coffea genetic
variability finding purpose, we require a Taq polymerase isolation from
recombinent E.coli. So, u suggest me  a  Taq polymerase isolation protocol
with buffer composition volumes.


          Im waiting for ur reply to me...

                                     Thanking u...
                                Satheesh Kumar
                           Junior Research Fellow,
                      Central Coffee Research Institute,
                                    India.


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