siRNA knockdown and MTT

[Scott Brown]

Hi,
 
I am attempting to knockdown a gene then put the cells into an MTT assay for 48 hrs. I need to the knockdown to last the length of the MTT assay.
I'm currently trying a 6hr siRNA transfection plated into 96 well plate, then treating with drug and measuring MTT response 48 hrs from that time point.
 
Can anyone suggest any "tricks" either with extending or enhancing knockdown effect or any ammended MTT protocols capable of producing a measurable response.
 
Many Thanks
 
S

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