Fwd: Methods Digest, Vol 40, Issue 19, Setting up a new mole biol laboratory

Shurjo Kumar Sen via methods%40net.bio.net (by shurjo.sen from gmail.com)
Fri Sep 26 15:17:23 EST 2008


A Nanodrop benchtop spectrophotometer is a good idea.

Also, check out the newest generation of transilluminators which use blue
light and are much safer than UV for both users and DNA. They work really
well (I just used one yesterday).
http://www.clarechemical.com/transilluminator.htm

---------- Forwarded message ----------
From: Virash Gupta <virashkgupta from gmail.com>
Date: 2008/9/26
Subject: Re: Methods Digest, Vol 40, Issue 19, Setting up a new mole biol
laboratory
To: methods from oat.bio.indiana.edu


Add a good quality laminar flow bench, simple PCR
mchines, dedicated microcentrifuge ets as well

On 9/25/08, methods-request from oat.bio.indiana.edu <
methods-request from oat.bio.indiana.edu> wrote:
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> Today's Topics:
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>   1. RE: Setting up a new mole biol laboratory (Rory O'Brien)
>   2. Transfection myoblast (laure d'astarac)
>   3. delipidized BCS (FBS)  (Zamawang Faisel Almemar-Ray)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 24 Sep 2008 11:26:58 +1200
> From: "Rory O'Brien" <rory.obrien from stonebow.otago.ac.nz>
> Subject: RE: Setting up a new mole biol laboratory
> To: <methods from magpie.bio.indiana.edu>
> Message-ID: <E1KiHHb-0007G8-L2 from galadriel.otago.ac.nz>
> Content-Type: text/plain;       charset="US-ASCII"
>
> A set of Current Protocols and/or the new(er) edition of Maniatis.
>
> and an ultrapure water supply, Milli-Q or similar.
>
> - R.
>
>
>
> > Dear Friends,
> >
> > We are planning to set up a molecular biology laboratory from
> > scratch. I am required to make a list of equipments required
> > for regular use in the laboratory. We have already
> > shortlisted major equipment like
> >
> > chemidoc system, RT PCR, 2D gel electrophoresis system,
> > laminar air flow,  etc
> >
> > with smaller equipments like submarine gel electrophoresis
> > units, PAGE units, western and southern blot apparatus, speed
> > vac, hand held uv torch, uv transilluminator, tabletop
> > centrifuge, autoclave,
> >
> > and other everyday use apparetus like waterbath, pH meter,
> > magnetic stirrers, vortex, incubator, oven, digital balance
> > and the freezers of -20 and -70 degrees Celsius etc
> >
> > I need some inputs on whether there is anything crucial from
> > the point of view of a mole biol lab that I may have missed
> > out... Any and all suggestions are welcome!
> >
> > Regards,
> > --Yg
> > _______________________________________________
> > Methods mailing list
> > Methods from net.bio.net
> > http://www.bio.net/biomail/listinfo/methods
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>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 24 Sep 2008 15:55:04 +0200
> From: "laure d'astarac" <lauredastarac from gmail.com>
> Subject: Transfection myoblast
> To: methods from magpie.bio.indiana.edu
> Message-ID:
>        <ba709e750809240655h4ce8e16cs62aa47498492d456 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
>
> I have to start transfection of AON and siRNA in my lab, where nobody has
> experience...
>
> So several questions about myoblast transfection with small RNAs (or
> AONs...)
>
> 1/ what is the best transfection method to use: Electroporation Vs
> Lipfectamin Vs other?
>
> 2/ If someone has general rules about minimum and maximum amount of AON
-Si
> RNA to use in transfection, in order to be efficient but not toxic... I
> suppose i have to do several quantities, but values are arround ng,
> microg???
>
> 3/ When harrest cells after transfection: 24h? 48? test both?
>
> I am sorry about these basic questions.... and thank you for your help....
>
> --
> Claire Navarro
> Stem cell laboratory
> University of Milan - Ospedale Maggiore Policlino di Milano -
> Padiglione Ponti, II piano
> Via F. Sforza, 35
> 20122 Milan
> Italy
> Tel: 00 39 02 55 03 38 52
> 00 39 02 55 03 38 74
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 23 Sep 2008 17:50:56 -0600
> From: "Zamawang Faisel Almemar-Ray" <zalmemar from uccs.edu>
> Subject: delipidized BCS (FBS)
> To: methods from magpie.bio.indiana.edu
> Message-ID: <web-80101311 from uccs.edu>
> Content-Type: text/plain;charset=iso-8859-1;format="flowed"
>
> Could someone please tell me how to make delipidized BCS (FBS) using
> charcoal stripping. I desparately need the procedure for this, as my
> research is on hold because my order for delipidized BCS (FBS) is on
> back-order.
>
> Thanks,
> Z
>
>
>
> ------------------------------
>
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> End of Methods Digest, Vol 40, Issue 19
> ***************************************
>



--
Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210
_______________________________________________
Methods mailing list
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-- 
**********************************************************
Shurjo Kumar Sen, Ph.D.

Postdoctoral Visiting Fellow
Genome Technology Branch
National Human Genome Research Institute
National Institutes of Health
Building 50, Room 5529
50 South Drive, MSC 8004
Bethesda, MD 20892-8004


Phone: 225-281-6808
e-mail: sensh from mail.nih.gov


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