retroviral vs lentiviral expression

[Aawara Chowdhury]

In <f09f9e4c-288c-4cf1-8c33-7fc72ed58071 from d77g2000hsb.googlegroups.com>,
 patrickzhang76 from gmail.com <patrickzhang76 from gmail.com> wrote:

> Hi, there. For the purpose of overexpressing a gene of interest in the
> dividing mammalian cells, is there any difference between using a
> retroviral systems and the newer lentiviral systems? Beside the
> obvious differences for non-dividing cells, what are the pros and cons
> between the two systems?

Retroviral vectors work extremely well for dividing cells.  There are
fewer viral proteins that need to be expressed, and typically support
larger inserts. Although MLV vectors are restricted to a total size
of about 8 kb, I've used Steve Hughes' avian retrovirus vectors with 
a total size of about 11 kb, of which about 8.5 kb was the insert ......

AC
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