Methods Digest

[Tom Knight]

"TieQiao Wu" <TieQiao.Wu from bakeridi.edu.au> writes:
> I am writing to you for getting your help. We recently tried to amplify
> a 1kb human genomic fragment with 85% GC content and tried different
> methods, however we failed to get products. Does anyone have any
> suggestion and advice? Thanks in advance.

I'd suggest adding 5-8% 1 molar betaine (Sigma) to your PCR reaction.
You could also try the Phusion high GC master mix (Finnzyme, NEB).