qPCR Newsletter September 2008

Editor www.Gene-Quantification.info via methods%40net.bio.net (by editor from gene-quantification.info)
Mon Sep 29 05:06:41 EST 2008

qPCR Newsletter September 2008

Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

- microRNA - new links and animations
- microRNA - updated microRNA application papers
- worldwide qPCR application workshops
- qPCR Symposium USA in November 2008 - register now !
- 1st announcement qPCR 2009 Event =3D> http://www.qPCR2009.net

New  http://www.Gene-Quantification.ASIA   pages online translation to

CHINESE =3D>  http://chinese.gene-quantification.asia/
JAPANESE =3D>  http://japanese.gene-quantification.asia/
KOREAN =3D>  http://korean.gene-quantification.asia/
RUSSIAN =3D>  http://russian.gene-quantification.asia/

Links on  http://microrna.gene-quantification.info/

microRNA Definition
non-coding RNAs / DataBase
micro RNA talks and microRNA posters
Nature Genetics 38, S1 (2006) - The microRevolution
miRNA Research Education Research Center
Human MicroRNA targets
miRU - Plant microRNA Potential Target Finder
Cancer genomics:   Small RNAs with big impacts
miRU: an automated plant miRNA target prediction server
miRBase - the home of microRNA data
miRBase: microRNA sequences, targets and gene nomenclature
The microRNA Registry
A uniform system for microRNA annotation
TRC - the RNAi Consortium shRNA Library
Algorithms & Thermodynamics for Nucleic Acid Folding, Hybridization &
Melting Profile Prediction
TUTORIAL:  Check out an illustrated tutorial on miRNA (by Ambion)


A lot of new application papers using microRNA quantitative real-time


Strategies to determine the biological function of microRNAs.
Genomics of microRNA.
Tracing microRNA patterns in mice.
Target mimics modulate miRNAs.
Target mimicry provides a new mechanism for regulation of microRNA
Lessons from Nature: microRNA-based shRNA libraries.
Identification of Differentially Expressed MicroRNAs by Microarray: A
Possible Role for MicroRNA Genes in Pituitary Adenomas.
RNA polymerase III transcribes human microRNAs.
Real-time expression profiling of microRNA precursors in human cancer
cell lines.
Multiplexing RT-PCR for the detection of multiple miRNA species in
small samples.
An oligonucleotide microchip for genome-wide microRNA profiling in
human and mouse tissues.
Microarray analysis shows that some microRNAs downregulate large
numbers of target mRNAs.
MicroRNA expression profiling using LNA-modified probes in a liquid-
phase bead-based array.
miRCURY=99 LNA research tools for microRNA.
Editorial - The microRevolution.
Glimpses of a Tiny RNA World.
Functions of microRNAs and related small RNAs in plants.
Evidence that microRNAs are associated with translating messenger RNAs
in human cells.
How microRNAs control cell division, differentiation and death.
microRNA target predictions in animals.
Human let-7a miRNA blocks protein production on actively translating
Computational identification of microRNA targets.
Intronic microRNA precursors that bypass Drosha processing.
MicroInspector: a web tool for detection of miRNA binding sites in an
RNA sequence.
Target labelling for the detection and profiling of microRNAs
expressed in CNS tissue using microarrays.
A brain-specific microRNA regulates dendritic spine development.


Update of the Webinar page

A lot of interesting TALKs, WEBINARs, SLIDE SHOWs, and PODCASTs from
various speakers, biotec companies, qPCR Events, and international
journals (Nature and Science) are FREE for download. Have a look and
you will definitely something interesting for your scientific work !


With the new qPCR INFO PORTAL and all the presented tools we will help
you with to find the right information about qPCR and related topics
in Molecular Biology in the literature and in the World Wide Web.
=3D>  Papers / Protocols / Methods / Databases / Alets / Feeds / Books /
Forums / E-mail / Directory



Upcoming Events World-wide academic and commercial qPCR Events

Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, ...etc..
Please submit your qPCR event here  =3D>  events from gene-



The prominent and still growing place taken by real-time quantitative
PCR in applied and fundamental research and clinical diagnostics
almost appears obvious. However, it is clear that contributions made
by various scientists and companies in the field during the last
decade rendered this technology useful and affordable for many users.

More info =3D>  http://www.gene-quantification.de/meetings.html#benelux

Importantly, the qPCR domain is still in constant evolution, making it
sometimes hard to stay informed about new methodological approaches or
original studies using the real-time PCR. Therefore, we have scheduled
a one day "Benelux qPCR Symposium" on October 6th 2008, giving the
opportunity to the scientific community to get informed and discuss
various aspects of real-time PCR (including but not limited to new
applications, assay optimization and validation, new technologies,
etc.). Scientific talks, posters sessions and industrial booths will
be at the menu.

Download poster =3D>  http://www.gene-quantification.de/qpcr_benelux_poster=


qPCR Symposium USA
10. - 13. November 2008
Clarion Hotel San Francisco Airport, Millbrae, CA , USA

More info =3D>  http://www.gene-quantification.de/meetings.html#qpcr_usa

- High throughput platforms:  High throughput applications, real-time
RT-PCR arrays, digital PCR
- Forthcoming technologies:  Immuno PCR, Methylation sensitive PCR,
SNP analysis, High resolution melt, microRNA detection, - Multiplex
- Single-cell qPCR:  Pre-amplification techniques, sub-cellular PCR,
Expression heterogeneity, laser microdissection, FACS sorting,
Enrichment of rare cells
- Multimarker diagnostics:  Disease markers, Tissue specific markers,
Cancer markers, Stem cells, Differentiation markers, Cancer stem cells
- Real-time PCR Expression Profiling:  multivariate and multiway
expression profiling, temporal expression profiling, spatiotemporal
- Pre-analytical Steps:  Sampling technologies, Extraction methods,
Reverse Transcription, Quality Control, Standards, Standard Operating
Procedures, Interlaboratory Exercises
- Normalization & Standardization:  Normalization strategies,
Reference genes, Spikes, Standard curves, multiplexing, inter-run
calibrators, quantification strategies, mRNA degradation
- Data management and data treatment:  software applications, data
mining, data visualization, biostatistics, multivariate statistics


qPCR 2009 Event

1st annoncement of the qPCR 2009 Event in Freising Weihenstephan


Download flyer =3D>  http://www.bioeps.com/qpcr2009/qPCR-2009-1st-announcem=



TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops,
the 3-day Core Module and a 2-day Biostatistics Module.  qPCR courses
are held in regularly in G=F6teborg, Sweden, in English and in Freising-
Weihenstephan, Germany, in German and English, and in Prague, Czech
Republic in English and Czech.
Depending on the occasion the workshop language and the different
prices may apply. Further customized workshops and specialized
trainings will be held as well across Europe and world-wide. TATAA
Biocenter Germany courses are held in cooperation with the Institute
of Physiology, located at the Technical University of Munich, in
Freising-Weihenstephan, near Munich, very close to the Munich Airport
(MUC). For more information and to register for the qPCR application
workshops, please see our web page:  http://tataa.gene-quantification.info/

Course Occasions summer and autumn 2008:

13-17 Oct Prague   RNA Isolation + qPCR Core Module + HRM
13-17 Oct Freising Germany   qPCR Core Module + Biostatistics (Kurs
wird in DEUTSCH gehalten, German language)
27-31 Oct G=F6teborg   qPCR Core Module + HRM + Biostatistics
17-21 Nov Prague   qPCR Core Module + Practical Biostatistics
24-28 Nov  Freising Germany    qPCR Core Module + Biostatistics
(English language)
1-5 Dec G=F6teborg    qPCR Core Module + Biostatistics
15-19 Dec Prague    RNA Isolation + Expression Profiling and Data
Download list of TATAA courses in autumn and fall 2008

Please register here  =3D>  http://www.tataa.com/Courses/Courses.html


Forward Please send the qPCR NEWS to further scientists and friends
who are interested in qPCR !

Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages


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