Chemically defined media required
(by davidminde from gmail.com)
Tue Apr 14 04:20:19 EST 2009
> Yet some refold very easily. It's always, always worth a shot.
> Easy to do, nothing to lose.
> I slightly disagree: For proteins where NO rock solid functional test is
> available for proper validation of refolding success, the risk of ending up
> with a heterogeneous population of partly refolded and partly misfolded
> protein is significantly increased by refolding (vs. e.g. soluble expression
> under cold-shock conditions) making it completely useless for most (ensemble
> based) biophysical and structure biology assays.
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