Expression and -N end rule

WS via methods%40net.bio.net (by novalidaddress from nurfuerspam.de)
Wed Apr 22 03:13:52 EST 2009


Hi Shifali, maybe your protein is toxic? you could change codon usage
and/or try higher eucaryotic cells.

To narrow down the effect of signal sequences etc.: Have you
considered putting your protein (i.e its cDNA) and/or part of it in
front of a reporter gene like GFP or insert just anything known in
order to disrupt a possile action?

Best regards,

Wo


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