Expression and -N end rule

[WS]

Hi Shifali, maybe your protein is toxic? you could change codon usage
and/or try higher eucaryotic cells.

To narrow down the effect of signal sequences etc.: Have you
considered putting your protein (i.e its cDNA) and/or part of it in
front of a reporter gene like GFP or insert just anything known in
order to disrupt a possile action?

Best regards,

Wo