Phenol in alcohol precip

[Barbara MacGregor]

Hi Ed,

As long as your DNA is precipitated, you can just spin it down; take  
off the supernatant; resuspend the DNA in water (or your choice of  
buffer); and then repeat the chloroform extraction, if you're worried  
there's still phenol there. You should get MOST of the DNA back.

Barbara

>
>
>
> From: Ed Siefker <ebs15242 from creighton.edu>
> Date: April 21, 2009 9:38:04 AM EDT
> To: methods from magpie.bio.indiana.edu
> Subject: phenol in alcohol precip
>
>
> Hi.  I was a little hasty in doing some phenol/chloroform
> preps of genomic DNA and apparently didn't remove all
> the phenol before adding ethanol to precipitate.  How can
> I recover from this?
>
> I considered extracting with chloroform again, but I don't
> know if the phases will still separate with ethanol in there.
> Also, the DNA isn't in solution, so I'm guessing it would
> collect at the interface.  If there were more DNA there,
> I'd just fish it out with a pipette tip, but it's a small prep.
>
> Any thoughts?  Thanks a bunch
> -Ed
>