fusion protein purification problem

Chris McDermott-Roe via methods%40net.bio.net (by cjmcdermottroe from googlemail.com)
Tue Aug 4 08:08:45 EST 2009


Hi all,

I have produced a myc-conjugated fusion protein and hope to express it in
mammalian cells and subsequently purify it.  Are there any myc resins out
there that would permit large-scale purification of my protein?  Second, if
I was to transfect my protein into say HEK293 cells, would I need to worry
about co-purifying endogenous m-Myc if using such a resin?

Thanks all

Chris


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