fusion protein purification problem
Chris McDermott-Roe
via methods%40net.bio.net
(by cjmcdermottroe from googlemail.com)
Tue Aug 4 08:08:45 EST 2009
Hi all,
I have produced a myc-conjugated fusion protein and hope to express it in
mammalian cells and subsequently purify it. Are there any myc resins out
there that would permit large-scale purification of my protein? Second, if
I was to transfect my protein into say HEK293 cells, would I need to worry
about co-purifying endogenous m-Myc if using such a resin?
Thanks all
Chris
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