Seeking method for fatty acid content determination in plant
seeds and seedlings
StewJW
via methods%40net.bio.net
(by stewjw from gmail.com)
Tue Aug 4 03:24:45 EST 2009
Some commercial lit. can contain some useful info particularly the
right type of GC column to use, although I would search the
sicientific lit. eg
http://www.sge.com/uploads/mF/aa/mFaaBmcFVcEMITZzKHgQmA/BR-0307-C.pdf
SGE recommend their BP21- Polyethylene Glycol (TPA treated) or a
BPX70-70% Cyanopropyl polysilphenylene-siloxane for the separation of
fatty acid methyl esters. There is also a cross-reference guide to
other manufacturer columns. Fisher UK who I work for also supply
Thermo Hypersil's Trace TR-WAX GC column for FA separation. Make sure
you get the right column for the job is the message, its a competative
field so you should be able to haggle a good price.
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