fusion protein purification problem
(by novalidaddress from nurfuerspam.de)
Tue Aug 4 12:53:42 EST 2009
You might consider immobilizing anti.myc antibodies on an activaed
sepharose (eg CNBr, NHS). Should not cross-react with endogenous myc.
On 4 Aug., 15:08, Chris McDermott-Roe <cjmcdermott... from googlemail.com>
> Hi all,
> I have produced a myc-conjugated fusion protein and hope to express it in
> mammalian cells and subsequently purify it. Are there any myc resins out
> there that would permit large-scale purification of my protein? Second, if
> I was to transfect my protein into say HEK293 cells, would I need to worry
> about co-purifying endogenous m-Myc if using such a resin?
> Thanks all
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