fusion protein purification problem

[WS]

Hi Chris,

You might consider immobilizing anti.myc antibodies on an activaed
sepharose (eg CNBr, NHS). Should not cross-react with endogenous myc.

Wo


On 4 Aug., 15:08, Chris McDermott-Roe <cjmcdermott... from googlemail.com>
wrote:
> Hi all,
>
> I have produced a myc-conjugated fusion protein and hope to express it in
> mammalian cells and subsequently purify it.  Are there any myc resins out
> there that would permit large-scale purification of my protein?  Second, if
> I was to transfect my protein into say HEK293 cells, would I need to worry
> about co-purifying endogenous m-Myc if using such a resin?
>
> Thanks all
>
> Chris