copy number determination real time vs sauthern blott

Emerson A. Castilho Martins via methods%40net.bio.net (by martinseac from gmail.com)
Sat Aug 22 15:44:57 EST 2009


In fact, Real Time PCR is much more sensitive than southern blot, but
you have to use standard curve for determination of this. 

Em Sáb, 2009-08-22 às 02:37 -0700, Azam Rahimpour escreveu:
> Dear All
> I am going to determine copy number of integrated genes in transfected cells, most articles used sauthern blotting for this purpose. Is it possible to use quantitative real time pcr instead of that? and is it as accurate as sauthern blotting in identification of single copies?
> best 
> 
> 
>       
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods



More information about the Methods mailing list