Expression vector selection
(by eenigoy from gmail.com)
Thu Dec 3 04:53:41 EST 2009
My colleagues are working on down stream processing of human G-CSF. The plan
includes scaling up at later stages.
They first plan to clone functional part of G-CSF gene in E. coli. This
part does not include the N terminal signal peptide and obviously the start
Can any one give me any suggestion about which expression system to go for?
They are particularly interested in vectors that
1. Have their own ATG
2. Are without His Tags etc. since these might interfere with the later DSP.
3. With a strong promoter.
Contact: +91 9223355677
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