pCD2 plasmid map
Peter Ellis
via methods%40net.bio.net
(by pjie2 from cam.ac.uk)
Fri Dec 4 16:48:22 EST 2009
Don't have a map to hand, but here's the fourth hit from a Google search
for "pCD2 sequence".
http://downloads.yeastgenome.org/sequence/vector/AF134166.gb
Given the sequence, working out a restriction map should be simple :-)
Peter
****************
LOCUS AF134166 6220 bp DNA SYN 30-DEC-1999
DEFINITION Yeast 2-hybrid vector pCD.2 complete sequence.
ACCESSION AF134166
VERSION AF134166.1 GI:6642844
KEYWORDS .
SOURCE Yeast 2-hybrid vector pCD.2.
ORGANISM Yeast 2-hybrid vector pCD.2
artificial sequence; vectors.
REFERENCE 1 (bases 1 to 6220)
AUTHORS Durfee,T., Draper,O., Zupan,J., Conklin,D.S. and Zambryski,P.C.
TITLE New tools for protein linkage mapping and general two-hybrid
screening
JOURNAL Yeast 15 (16), 1761-1768 (1999)
MEDLINE 20060013
PUBMED 10590464
REFERENCE 2 (bases 1 to 6220)
AUTHORS Durfee,T., Draper,O. and Zupan,J.
TITLE Direct Submission
JOURNAL Submitted (11-MAR-1999) Plant and Microbial Biology, UC
Berkeley,
111 Koshland Hall, Berkeley, CA 94720, USA
FEATURES Location/Qualifiers
source 1..6220
/organism="Yeast 2-hybrid vector pCD.2"
/db_xref="taxon:111294"
rep_origin 1..362
/note="ColE1 replication origin cloned from pRS314;
Sikorski, RS and Hieter, P. 1989"
gene complement(934..1794)
/gene="bla"
CDS complement(934..1794)
/gene="bla"
/note="confers ampicillin resistance; cloned from
pRS314;
Sikorski, RS and Hieter, P. 1989"
/codon_start=1
/product="beta-lactamase"
/protein_id="AAF20321.1"
/db_xref="GI:6642845"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
misc_feature 2028..2053
/note="CEN6: CDEI fragment of chromosome VI centromere
from S.cerevisiae; cloned from pRS314; Sikorski,
RS and
Hieter, P. 1989"
misc_feature 2066..2442
/note="ARS4: Autonomous replicating sequence fragment
near gene for histone 4 on chromosome II in S.
cerevisiae;
cloned from pRS314; Sikorski, RS and Hieter, P. 1989"
gene 2976..3650
/gene="TRP1"
CDS 2976..3650
/gene="TRP1"
/note="cloned from pRS314; Sikorski, RS and Hieter, P.
1989"
/codon_start=1
/product="n-(5'-phosphoribosyl)-anthranilate
isomerase"
/protein_id="AAF20322.1"
/db_xref="GI:6642846"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK"
promoter 4437..5165
/note="ADC1 promoter (PADH) cloned from pAS1;
Durfee, T.
1993"
CDS 5166..>5607
/note="binds UASG site cloned from pAS1; Durfee, T.
1993."
/codon_start=1
/product="GAL4 DNA binding domain"
/protein_id="AAF20323.1"
/db_xref="GI:6642847"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKT
KRSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNV
NKDAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
misc_feature 5661..5700
/note="hemagglutinin epitope cloned from pAS1;
Durfee, T.
1993"
misc_feature 5661..5730
/note="multiple cloning site (NdeI, SfiI, NcoI, SmaI,
BamHI, SalI, PstI) cloned from pAS1; Durfee, T. 1993"
terminator 5748..5938
/note="ADC1 transcriptional terminator (TADH)
cloned from
pAS1; Durfee, T. 1993."
BASE COUNT 1748 a 1353 c 1417 g 1702 t
ORIGIN
1 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
61 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
121 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
181 taggctcggc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
241 cccgacagga ctataaagat accaggcgtt cccccctgga agctccctcg tgcgctctcc
301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
361 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
601 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
721 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
781 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
841 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
901 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
961 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactgcccg tcgtgtagat
1021 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
1081 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
1141 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
1201 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
1261 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
1321 agttacatga tcccccatgt tgtgaaaaaa agcggttagc tccttcggtc ctccgatcgt
1381 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
1441 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
1501 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
1561 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
1621 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
1681 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
1741 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
1801 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
1861 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
1921 acctgggtcc ttttcatcac gtgctataaa aataattata atttaaattt tttaatataa
1981 atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt
2041 ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta tcttgctctt
2101 cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaag accacacacg
2161 aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta tttaatctgc
2221 ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt tttaaacctt
2281 tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac tttctaaaat
2341 ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta ttccatcatt
2401 aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtcctaagaa accattatta
2461 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
2521 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
2581 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
2641 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac cataaacgac
2701 attactatat atataatata ggaagcattt aatagacagc atcgtaatat atgtgtactt
2761 tgcagttatg acgccagatg gcagtagtgg aagatattct ttattgaaaa atagcttgtc
2821 accttacgta caatcttgat ccggagcttt tctttttttg ccgattaaga attaattcgg
2881 tcgaaaaaag aaaaggagag ggccaagagg gagggcattg gtgactattg agcacgtgag
2941 tatacgtgat taagcacaca aaggcagctt ggagtatgtc tgttattaat ttcacaggta
3001 gttctggtcc attggtgaaa gtttgcggct tgcagagcac agaggccgca gaatgtgctc
3061 tagattccga tgctgacttg ctgggtatta tatgtgtgcc caatagaaag agaacaattg
3121 acccggttat tgcaaggaaa atttcaagtc ttgtaaaagc atataaaaat agttcaggca
3181 ctccgaaata cttggttggc gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg
3241 tcaatgatta cggcattgat atcgtccaac tgcatggaga tgagtcgtgg caagaatacc
3301 aagagttcct cggtttgcca gttattaaaa gactcgtatt tccaaaagac tgcaacatac
3361 tactcagtgc agcttcacag aaacctcatt cgtttattcc cttgtttgat tcagaagcag
3421 gtgggacagg tgaacttttg gattggaact cgatttctga ctgggttgga aggcaagaga
3481 gccccgaaag cttacatttt atgttagctg gtggactgac gccagaaaat gttggtgatg
3541 cgcttagatt aaatggcgtt attggtgttg atgtaagcgg aggtgtggag acaaatggtg
3601 taaaagactc taacaaaata gcaaatttcg tcaaaaatgc taagaaatag gttattactg
3661 agtagtattt atttaagtat tgtttgtgca cttgcctgcg gtgtgaaata ccgcacagat
3721 gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc
3781 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
3841 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
3901 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
3961 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
4021 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
4081 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
4141 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
4201 gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
4261 ttcgctatta cgccagctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac
4321 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
4381 actatagggc gaattggagc tccaccgcgg tggcggccgc tctagaacta gatatccttt
4441 tgttgtttcc gggtgtacaa tatggacttc ctcttttctg gcaaccaaac ccatacatcg
4501 ggattcctat aataccttcg ttggtctccc taacatgtag gtggcggagg ggagatatac
4561 aatagaacag ataccagaca agacataatg ggctaaacaa gactacacca attacactgc
4621 ctcattgatg gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc
4681 atatcgaagt ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat
4741 gcaacttctt ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc
4801 aatgacaaaa aaaatgatgg aagacactaa aggaaaaaat taacgacaaa gacagcacca
4861 acagatgtcg ttgttccaga gctgatgagg ggtatcttcg aacacacgaa actttttcct
4921 tccttcattc acgcacacta ctctctaatg agcaacggta tacggccttc cttccagtta
4981 cttgaatttg aaataaaaaa agtttgccgc tttgctatca agtataaata gacctgcaat
5041 tattaatctt ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct
5101 gcacaatatt tcaagctata ccaagcatac aatcaactcc aagcttgaag caagcctcct
5161 gaaagatgaa gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc
5221 tcaagtgctc caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc
5281 gctactctcc caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat
5341 caaggctaga aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca
5401 tgattttgaa aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac
5461 aagataatgt gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc
5521 ctctaacatt gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca
5581 aaggtcaaag acagttgact gtatcgccgg aattcatggc ttacccatac gatgttccag
5641 attacgctag cttgggtggt catatggcca tggacctagg cagatctgat caagggatcc
5701 cgggtcgacc tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt
5761 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
5821 aaacgaaaat tcttgttctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
5881 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcgacgtcg ggcccggtac
5941 ccagcttttg ttccctttag tgagggttaa ttccgagctt ggcgtaatca tggtcatagc
6001 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatagga gccggaagca
6061 taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt gcgttgcgct
6121 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
6181 gcgcggggag aggcggtttg cgtattgggc gctcttccgc
//
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