Separating PCR products on agarose gel with similar bp length

Taliaferro, Dwayne (NIH/NIMH) [F] via methods%40net.bio.net (by taliaferrod from mail.nih.gov)
Fri Dec 4 17:26:31 EST 2009


Run a larger gel (more distance between the + and - ends) overnight with 3% agarose.  You could also try a polyacrylamide gel as well, but I don't have experience running one for that purpose.  If its appropriate I'm sure someone will suggest the proper % and setup apparatus.


On 12/4/09 4:17 PM, "umwong25 from cc.umanitoba.ca" <umwong25 from cc.umanitoba.ca> wrote:

Hi,

I am trying to separate 745bp, 646bp, and 655bp fragments on an
agarose gel. I've separated the 745bp fragment but the other two
fragments are not separating on a 2.5% agarose gel, running at 55V for
3.5 hours. I also want a crisp band because I am planning to cut these
fragments out and extract the DNA for sequencing. Any suggestions?


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