Cloning trick

Nasser Mahna via (by n.mahna from
Tue Dec 22 12:23:10 EST 2009

Dear All,
I would to ask if there is anybody mastered in working with
restriction and digestion.
I want to clone a pcr fragment inside a binary vector. To do it
efficiently, I want to use a restriction enzyme after ligating vector
and fragment, which cuts the original uncut vector before
what method do you offer?
Best regards,

More information about the Methods mailing list