Cloning trick

Nasser Mahna via methods%40net.bio.net (by n.mahna from gmail.com)
Tue Dec 22 12:23:10 EST 2009


Dear All,
I would to ask if there is anybody mastered in working with
restriction and digestion.
I want to clone a pcr fragment inside a binary vector. To do it
efficiently, I want to use a restriction enzyme after ligating vector
and fragment, which cuts the original uncut vector before
transformation.
what method do you offer?
Best regards,
Nasser.



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