non fluorecent way for nuclear staining

Nick Theodorakis via methods%40net.bio.net (by nick.theodorakis from gmail.com)
Mon Dec 28 09:10:41 EST 2009


On Dec 27, 9:51 am, Sébastien Vigneau <sebastien.vign... from gmail.com>
wrote:
> Haematoxylin?

I was thinking of that, too.

Some clarification from the OP would help, too. Is it that you don't
have access to a fluorescent scope, or that you don't want the stain
to be fluorescent? Because if it is the latter, I believe that
hematoxylin is autofluorescent; or at least, some component of an H&E
stained slide is autofluorescent.

What is the nature of your sample? Live, fixed or frozen? Cells or
tissues? How are you visualizing it now?

Nick

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