non fluorecent way for nuclear staining

Sébastien Vigneau via methods%40net.bio.net (by sebastien.vigneau from gmail.com)
Mon Dec 28 14:38:02 EST 2009


Maybe Nile Blue, or Neutral
Red<http://www.histosearch.com/histonet/Jul00A/Re.fastredneutralornuclea.html>
.

Sébastien Vigneau


On Mon, Dec 28, 2009 at 1:40 PM, merav karsenty <meravkr from gmail.com> wrote:

> I need the stain to be non fluorecent because I dont have acess to a
> fluorecent microscop. I know Haematoxylin, but as I anderstand, using this
> stain must involve fixation. I am working with living  cells and I am
> trying
> to find a way to stain them without fixation, unless there is no choice. Is
> there a way that you know?
>
> 2009/12/28 Nick Theodorakis <nick.theodorakis from gmail.com>
>
> > On Dec 27, 9:51 am, Sébastien Vigneau <sebastien.vign... from gmail.com>
> > wrote:
> > > Haematoxylin?
> >
> > I was thinking of that, too.
> >
> > Some clarification from the OP would help, too. Is it that you don't
> > have access to a fluorescent scope, or that you don't want the stain
> > to be fluorescent? Because if it is the latter, I believe that
> > hematoxylin is autofluorescent; or at least, some component of an H&E
> > stained slide is autofluorescent.
> >
> > What is the nature of your sample? Live, fixed or frozen? Cells or
> > tissues? How are you visualizing it now?
> >
> > Nick
> >
> > --
> > Nick Theodorakis
> > nick_theodorakis from hotmail.com
> > contact form:
> > http://theodorakis.net/contact.html
> > _______________________________________________
> > Methods mailing list
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> >
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