(by aawara from pontiff-playground.org)
Sun Feb 1 17:06:18 EST 2009
In <6udb0pFeoar3U1 from mid.individual.net>,
Christian Praetorius <prae from gmx.net> wrote:
> dk from no.email.thankstospam.net (DK) wrote:
>>The best and easiest thing is to do nothing. 1 ul of ligation mix
>>is perfectly OK with normal electroporation.
> Thats my experience, too. Making too much effort doesn't make sense.
Oddly enough this has failed for me - many, many, times. We use 1 mm gap
cuvettes, and 1800 volts (25 uF capacitance, and I think 200 ohms
resistance). What are your settings?
I would love to be able to do away with drop dialysis.
Email: echo 36434455860060025978157675027927670979097959886449930P | dc
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