Electroporation

Aawara Chowdhury via methods%40net.bio.net (by aawara from pontiff-playground.org)
Sun Feb 1 17:06:18 EST 2009


In <6udb0pFeoar3U1 from mid.individual.net>,
 Christian Praetorius <prae from gmx.net> wrote:

> dk from no.email.thankstospam.net (DK) wrote:
>
>>The best and easiest thing is to do nothing. 1 ul of ligation mix
>>is perfectly OK with normal electroporation. 
>
> Thats my experience, too. Making too much effort doesn't make sense.

Oddly enough this has failed for me - many, many, times.  We use 1 mm gap
cuvettes, and 1800 volts (25 uF capacitance, and I think 200 ohms 
resistance).  What are your settings?

I would love to be able to do away with drop dialysis.

AC
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