Electroporation

Aawara Chowdhury via methods%40net.bio.net (by aawara from pontiff-playground.org)
Mon Feb 2 06:27:21 EST 2009


In <6unrddFg2336U1 from mid.individual.net>,
 Christian Praetorius <prae from gmx.net> wrote:

> Aawara Chowdhury <aawara from pontiff-playground.org> wrote:
>
>>Oddly enough this has failed for me - many, many, times.  We use 1 mm gap
>>cuvettes, and 1800 volts (25 uF capacitance, and I think 200 ohms 
>>resistance).  What are your settings?
>
> I used the same settings, when we did electroporation. As long as the
> cells were washed good enough, we never had problems with short
> circuits. Since we have a quite good protocol for making
> supercompetent cells, we moved away from it.

Can you share this protocol with us?

Thanks!
AC

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