STABILIZERS FOR ENZYMES (mainly XYLANASE)Methods Digest, Vol 45, Issue 7

whyharish reddy via methods%40net.bio.net (by whyharish from gmail.com)
Wed Feb 11 14:29:15 EST 2009


Hello ,
This is HARISH KUMAR REDDY Y,
*MY PROBLEM:*
*I am unable to maintain my enzyme activity not more than 2 days, *
*please help in mainatining the activity of XYLANASE ENZYME for more than
2-3 months, suggest some Enzyme Stablizers?*
**
*thanking you *
**
*Harish Kumar Reddy Y*
From
GLOBAL INSTITUTE OF BIOTECHNOLOGY
 &
 OSMANIA UNIVERSITY, HYDERABAD, INDIA .
**


On Mon, Feb 9, 2009 at 10:34 PM, <methods-request from oat.bio.indiana.edu>wrote:

> Send Methods mailing list submissions to
>        methods from net.bio.net
>
> To subscribe or unsubscribe via the World Wide Web, visit
>        http://www.bio.net/biomail/listinfo/methods
> or, via email, send a message with subject or body 'help' to
>        methods-request from net.bio.net
>
> You can reach the person managing the list at
>        methods-owner from net.bio.net
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Methods digest..."
>
>
> Today's Topics:
>
>   1. Re: Electroporation (Kyle Legate)
>   2. Re: Bacterial growth measurement at 600nm? (Yoram Gerchman)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 08 Feb 2009 11:16:13 +0100
> From: Kyle Legate <legatekBLAH from hotmail.com>
> Subject: Re: Electroporation
> To: methods from net.bio.net
> Message-ID: <6v7pmrFiollvU1 from mid.individual.net>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Christian Praetorius wrote:
>
> >> for a little longer at 37C seems to work just as well as 42C. Saves
> >>from paranoia in the lab about someone "absolutely needing" 42C
> >> bath NOW :-)
> >
> > :-)
> > The heat shocking is something religious anyway. Ask two and they will
> > tell you at least three different times for the heat shock. I found
> > anything between 30sec and 2min working.
> >
> Heat shock is not even necessary in most cases. It depends on the
> strain, mostly. In my hands, SURE cells require a heat shock but
> DH5alpha do not. Since I use DH5alpha these days for cloning I only used
> heat shock for a particularly troublesome blunt ligation that was rather
> inefficient (heat shock does raise the transformation efficiency by
> about an order of magnitude, but since my competent cells are often
> ~10^9 I don't need it). My protocol:
>
> 1. Incubate DNA mix (ligation, supercoil, etc) with E. coli 5-10 minutes
> on ice.
> 2. Add 10X vol room temp LB and directly plate ~30 uL (for supercoil) or
> 100 uL (for ligation).
> 3. ???
> 4. Profit!
>
> Kyle
>
>
> ------------------------------
>
> Message: 2
> Date: Sun,  8 Feb 2009 22:13:48 +0200
> From: Yoram Gerchman <gerchman from research.haifa.ac.il>
> Subject: Re: Bacterial growth measurement at 600nm?
> To: methods from oat.bio.indiana.edu
> Message-ID: <1234124028.498f3cfce8802 from webmail.haifa.ac.il>
> Content-Type: text/plain; charset=ISO-8859-1
>
>
> This might help. Cyanobacteria growth is often measured at 750nm due to
> pigments
> absorbent at 600nm.
> Yoram
>
> ------------------------------------------------------------------------
> This message was sent using IMP, the Webmail Program of Haifa University
>
>
>
> ------------------------------
>
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>
> End of Methods Digest, Vol 45, Issue 7
> **************************************
>



-- 
Y.HARISH KUMAR REDDY


More information about the Methods mailing list