qPCR NEWS January 2009 - update in available real-time PCR cycler

Editor www.Gene-Quantification.info via methods%40net.bio.net (by editor from gene-quantification.info)
Thu Feb 19 06:04:47 EST 2009

qPCR NEWS January 2009 - update in available real-time PCR cycler

Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

- qPCR 2009 Event   =3D> http://www.qPCR2009.net
- we are proud to present 230 scientific contributions (86 talks & 144
posters) have a look on the preliminary online agenda =3D>
- real-time PCR Cycler page updated =3D> http://cyclers.gene-quantification=
- webinar page updated =3D> http://webinar.gene-quantification.info/
- Online translation service of the Gene Quantification =3D>

New qPCR workshop modules at the TATAA Biocenter Germany =3D>
European wide qPCR application workshops =3D>  register now !
=3D> course program spring - summer 2009
=3D> http://www.gene-quantification.de/bioeps-courses-spring-summer-2009.pd=


real-time PCR Cycler

On this page the most prominent real-time PCR cycler are described.

In the cycler descriptions the specifications and the advantages of
the displayed systems are shown. Which real-time platform meets your
requirements best, depends on your research application. Some of the
systems are designed for research with low capacities and others are
for high-throughput applications, most in combination with pipetting
robots. Most of them use solid-block for thermal cycling, other use
hot- and cooled-air. Most differences are obviously in the application
software, especially in the way of data analysis and how the derived
crossing points or threshold levels are computed. Each of these
systems employs either one of several general types of fluorescent
probes for detection. There are also big differences how data are
displayed. Some of the limitations of end-point detection in (RT-) PCR
have been assuaged in real-time PCR systems, a number of which are now
on the market. These systems offer many general technical advantages,
including reduced probabilities of variability and contamination, as
well as online monitoring and the lack of need for post reaction
analyses. Further, some of these systems were developed with
contemporary applications such as quantitative PCR, multiplexing, and
high-throughput analysis in mind. Initial template levels can be
calculated by analysing the shape of the curve or by determining when
the signal rises above some threshold value. Several commercially
available real-time PCR systems are overviewed on this page and/or
summarized in the accompanying table.
=3D> http://cyclers.gene-quantification.info/


Real-Time PCR: Current Technology and Applications

Editor: Julie Logan, Kirstin Edwards and Nick Saunders Applied and
Functional Genomics, Health Protection Agency, London (2009)  ISBN:
9781904455394    Publisher: Caister Academic Press

Chapter 4 - Reference Gene Validation Software for Improved
J. Vandesompele, M. Kubista and M. W. Pfaffl  (2009)

Real-time PCR is the method of choice for expression analysis of a
limited number of genes. The measured gene expression variation
between subjects is the sum of the true biological variation and
several confounding factors resulting in non-specific variation. The
purpose of normalization is to remove the non-biological variation as
much as possible. Several normalization strategies have been proposed,
but the use of one or more reference genes is currently the preferred
way of normalization. While these reference genes constitute the best
possible normalizers, a major problem is that these genes have no
constant expression under all experimental conditions. The
experimenter therefore needs to carefully assess whether a certain
reference gene is stably expressed in the experimental system under
study. This is not trivial and represents a circular problem.
Fortunately, several algorithms and freely available software have
been developed to address this problem. This chapter aims to provide
an overview of the different concepts.

Chapter 5 - Data Analysis Software
M. W. Pfaffl, J. Vandesompele and M. Kubista  (2009)

Quantitative real-time RT-PCR (qRT-PCR) is widely and increasingly
used in any kind of mRNA quantification, because of its high
sensitivity, good reproducibility and wide dynamic quantification
range. While qRT-PCR has a tremendous potential for analytical and
quantitative applications, a comprehensive understanding of its
underlying principles is important. Beside the classical RT-PCR
parameters, e.g. primer design, RNA quality, RT and polymerase
performances, the fidelity of the quantification process is highly
dependent on a valid data analysis. This review will cover all aspects
of data acquisition (trueness, reproducibility, and robustness),
potentials in data modification and will focus particularly on
relative quantification methods. Furthermore useful bioinformatical,
biostatical as well as multi-dimensional expression software tools
will be presented.


Webinars  in  qPCR  &  RNAinterference  &  Molecular-Biology

Here you find a huge summary of webinars in the field of qPCR &
RNAinterference & Molecular-Biology
=3D> http://webinar.gene-quantification.info/

online translation

Since October 2008 we provide an online translation service of the
Gene Quantification pages to several languages. Please recognize this
is an automatic and robotic based translation service, and therefore
we can give NO guarantee about the generated content. It should help
to understand the "rough" content of the Gene Quantification pages,
but still the original is the ENGLISH version:




With the new qPCR INFO PORTAL and all the presented tools we will help
you with to find the right information about qPCR and related topics
in Molecular Biology in the literature and in the World Wide Web.
=3D>  Papers / Protocols / Methods / Databases / Alets / Feeds / Books /
Forums / E-mail / Directory



Upcoming Events World-wide academic and commercial qPCR Events

Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, etc.
Please submit your qPCR event here  =3D>  events from gene-


qPCR 2009 EVENT - 9 - 13 March 2009

more news here  =3D>  http://www.qPCR2009.net
download event FLYER  =3D>  http://www.bioeps.com/qpcr2009/qPCR-2009-3rd-an=

We are proud to present 230 scientific contributions (86 talks & 144
posters) have a look on the preliminary online agenda =3D>

It is a pleasure to announce the Nobel Prize Laureate Kary Mullis at
the qPCR 2009 event in an own session =9325th Anniversary of PCR=94

List of confirmed speakers  =3D>  http://speakers.qpcr2009.net/
An industrial exhibition with the 30 world leading companies will be
held during the qPCR Symposium March 9-11  =3D>  http://exhibition.qpcr2009=

Our sponsors  =3D>  http://sponsors.qpcr2009.net/

Please register until 31st December to get the early registartion fee
=3D>  http://registration.qpcr2009.net/

Keynote lectures

The Pioneer in PCR   Kary B. Mullis   1993 Nobel Prize in Chemistry
- 25th Anniversary of PCR

Stephen A. Bustin
Professor of Molecular Science, Institute of Cell and Molecular
Science, Queen Mary's School of Medicine and Dentistry, University of
London, UK

- A new qPCR assay for the detection of Clostridium difficile
- MIQE- guidelines for publication of qPCR data

Ken Livak
Senior Scientific Fellow, Fluidigm Corporation,San Francisco, CA, US
- Moving from qPCR Assays to qPCR Arrays.



BioEPS GmbH / TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops, a
3-day qPCR Core Module and a 2-day qPCR Biostatistics Module.  All
courses are held regularly in G=F6teborg, Sweden, in English and in
Freising-Weihenstephan, Germany, in German and English, and in Prague,
Czech Republic in English and Czech.
Depending on the occasion the workshop language and the different
prices may apply. Further customized workshops and specialized
trainings will be held as well across Europe and world-wide.
TATAA Biocenter Germany workshops are held in cooperation with BioEPS
GmbH, located at the campus of the Technical University of Munich, in
Freising-Weihenstephan, very close to the Munich Airport (MUC). For
more information and registration, please see our web page:
=3D> http://TATAA.gene-quantification.info/

Course Occasions 2009:

3-day qPCR Core Module  (Mon. - Wed.)
2-day BioStatistics Module (Thu. - Fri.)
3-day single-cell qPCR Module  (Mon. - Wed.)
3-day microRNA Module   (Mon. - Wed.)

20 - 22 April 2009  (E)      NEW microRNA qPCR

27 - 29 April 2009  (E) NEW singel-cell qPCR Application Workshop

11 - 15 May 2009 (Deutsch)
3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module
(Thu. - Fri.)

15 - 19 Juni 2009  (E)
3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module
(Thu. - Fri.)

13 - 15 Juli 2009  (E)         NEW microRNA qPCR

27 - 31 Juli 2009  (E)
3-day qPCR Core Module (Mon. - Wed.) & 2-day BioStatistics Module
(Thu. - Fri.)

=3D> http://www.gene-quantification.de/bioeps-courses-spring-summer-2009.pd=


Forward Please send the qPCR NEWS to further scientists and friends
who are interested in qPCR !

Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages


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