Regarding Plasmid Expression

Sudheendra Rao N R via methods%40net.bio.net (by sudhee26 from gmail.com)
Thu Feb 26 03:35:31 EST 2009


Dear all,I have been working with a gene cloned into pcDNA 3.1 V5/His
vector. However, over time the expression has decreased drastically.
However, another gene cloned into same vector has consistently high
expression, till now (that rules out cell problems too). I did prepare fresh
Midi stock..but problem remains. What has gone wrong?


faithfully
sudheendra.

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