vector ligation problem
(by huazhongw from hotmail.com)
Thu Feb 26 09:32:50 EST 2009
I am working with vector constuction. I inserted a fragment into a vector by routine single-digest \electrophoresis\recovery\ligation\tranformation method. When I recut recombinant plasmids with the same enzyme, I run into a problem that the vector fragment size become smaller than the original cut vector.
I don't know why and need help!
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