vector ligation problem

Sudheendra Rao N R via (by sudhee26 from
Thu Feb 26 10:57:15 EST 2009

Hi ,Check for the star activity of the enzyme you are using.
Also following links might be useful to you.


On Thu, Feb 26, 2009 at 8:02 PM, huazhongw <huazhongw from> wrote:

> Dear memebers,
>       I am working with  vector constuction. I inserted a fragment into a
> vector by routine single-digest
> \electrophoresis\recovery\ligation\tranformation method. When I recut
> recombinant plasmids with the same enzyme, I run into a problem that the
> vector fragment size become smaller than the original cut vector.
>       I don't know why and need help!
> Huazhong Wang
> _______________________________________________
> Methods mailing list
> Methods from

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