pHIS2.1 one-hybrid vector request

fboernke from biologie.uni-erlangen.de via methods%40net.bio.net (by fboernke from biologie.uni-erlangen.de)
Fri Jan 9 08:43:03 EST 2009


Dear all,

I'd like to do a yeast one-hybrid screen to indentify proteins binding
to a specific DNA fragment. I've got all the neccesary components
except the pHIS2.1 reporter vector which is part of clontech's
matchmaker yeast one
hybrid kit. Unfortunately, they do not sell this plasmid separately.
Does anybody have this vector and would
be willing to  share an aliquot?
Thanks in advance
Ricky


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