(by molecular from khayam.ut.ac.ir)
Sun Jan 11 08:04:59 EST 2009
Dear Dr.Zbigniew Rudzki
I'm Mohadese Dastpeyman,MSC student of molecular cell biology at tehran
university,I saw your direction to Sebastien PARIS about adhesion assay at
as you are well experienced in adhesion assay would you please help me?
My thesis is about effect of a herbal drug on metastasis and invasion
properties of MDA-MB-231 breast cancer cells.
I want to test cell adhesion assay for this cells with collagen type 2.
now ,as you regarded before,there are a lot of protocols and I 'm wondering
between two of them about counting of the remaining cells.some prptpcpls
suggested adding MTT,and some of them suggested fixing cells with christal
violet and then lysed cells with triton x 100 ,then read the absorbance at
your suggestion is important to me,what do you thimk ?which of them is
is there any protocol that you accepted it as a standardize protocol?
Department of molecular cell biology
Tehran University of Sciences,
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