pI determination methods for QC

[Lori Kovatch]

Hi,

 

I am currently attempting to bring IEF testing in house for our Quality
Control lab.  We have two proteins that we need to be able to determine
pI.  I know around what the pI should be as we have sent them to a
contract lab for testing previously.  One of the proteins has a rather
high pI around 11. I thought I could use Invitrogen's IPG strips with
the Zoom Runner system,  but I am unsure of how to actually determine
the pI once I have run the system.  Now from what I understand,  their
system is more a tool for a separation of proteins and not for the
determination of pI.  Does anyone know how I could determine or if I can
determine pI using this system?

 

Thanks

 

Lori Kovatch Long

Quality Control Manager

Kentucky BioProcessing, LLC

3700 Airpark Drive  Owensboro, KY  42301

ph 270-663-6132   fax 270-689-2571