Black hole quenchers (BHQ) and 3' blocking in Real-time PCR
(by ivanoov from gmail.com)
Thu Jan 15 03:08:59 EST 2009
I am relatively new in the Real-time PCR world. I am about to order a
bunch of hydrolysis probes quenched with the BHQ.
The company states exclusively that they can do 3' - BHQ labeling but
is this also mean that the 3'-OH will be blocked by the quencher
against extension or I must order 3' phosphorylation in addition.
Any suggestions will be most welcome...
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