Black hole quenchers (BHQ) and 3' blocking in Real-time PCR

chovek69 via methods%40net.bio.net (by ivanoov from gmail.com)
Thu Jan 15 03:08:59 EST 2009


Dear experts,

I am relatively new in the Real-time PCR world.  I am about to order a
bunch of hydrolysis probes quenched with the BHQ.

The company states exclusively that they can do 3' - BHQ labeling but
is this also mean that the 3'-OH will be blocked by the quencher
against extension  or I must order 3' phosphorylation in addition.

Any suggestions will be most welcome...
Thanks
Ivan


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