Low concentration miniprep

Fulvio Celsi via methods%40net.bio.net (by fulvio.celsi from gmail.com)
Thu Jan 15 06:46:31 EST 2009


Hi Yasir
is your gene very large?what kit are you using? if the gene (and so the
plasmid) is large, maybe you should try to increase the quantity of the
bacteria when doing miniprep...

2009/1/14 muhammad yasir <yasirphr from yahoo.com>

> I have clone the gene in pUC19 and get conformation with restriction
> digestion. But unfortunately, I get very low quantity of plasmid after
> miniprep. While the control (circular pUC19) concentration is quite enough
> with miniprep kit that I used. I shall be very glad to know about the
> possible reason of low concentration and how can I improve the yield of
> clone plasmid from miniprep.
>
> Yasir
>
>
>
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-- 
Fulvio Celsi
BsC,PhD
Sector of Neurobiology,
International School for Advanced Studies, Scuola Internazionale di Studi
Superiori Avanzati (SISSA),
Trieste
Italy

Mobile: +393286489131


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